Document Type


Publication Date

Winter 1-5-2023


Aim: to observe the effect of propolis and its nano counterpart when used as final irrigation materials and compare them to the conventionally used EDTA and saline on the clinical outcomes of necrotic mature anterior teeth with apical periodontitis after treating them using regenerative endodontic procedure.

Methodology: 84 patients who fulfilled the inclusion criteria were recruited for the study. Access cavity was prepared, rubber dam isolation was done. Mechanical preparation was performed up to #60 using K-files. Final irrigation procedure was done as follows:

Group A (Control ): Final irrigation was done using saline (3ml/min) followed by 17% EDTA solution (20 mL/canal, 5 min).

Group B (comparator 1): Final irrigation was done using propolis[1] irrigation (20 mL/canal, 5 min).

Group C (comparator 2): Final irrigation was done using Nano-propolis[2] irrigation (20 mL/canal, 5 min).

Bleeding was then induced, PRF was placed in the canals, followed by Collacote[3] and Bio-dentine[4] placement. Patients were recalled after 6 and 12 months. Teeth were checked for sensibility using electric pulp tester and thermal tests. All data was analysed using Kruskal Wallis test.

Results: All of the 84 patients (100%) within the 3 groups showed both clinical success and survival. Using Kruskal Wallis, there was no statistically significant difference among the three groups (p=1). Two teeth in the nano propolis group responded positively to thermal test at 12 months follow up. None of the teeth in the three tested groups (100%) responded to the

electric pulp test at 12 months. There was nostatistically significant difference between the three groups(p=1)

Conclusion: within the limitation of the study, single visit regenerative endodontics can be used to treat necrotic mature teeth with apical periodontitis and a periapical lesion.