Polyphenols LC-MS2 profile of Ajwa date fruit (Phoenix dactylifera L.) and their microemulsion: Potential impact on hepatic fibrosis
© 2018 Ajwa fruits are among the most nutritious and economically valuable fruits. In this study, the effect of different solvents on the extraction of polyphenols was investigated. Aqueous acetone (50%) extract contained the highest content of polyphenols and flavonoids. Filtration of the extract through a Diaion HP-20 column led to a polyphenols-enriched fraction (Fr 1) that had the highest antioxidant activity as measured with ORAC and DPPH assays. Metabolic profiling of the different extracts using HPLC-ESI-QTOF-MS-MS led to the tentative identification of 33 secondary metabolites: 3 phenolic acids, 19 flavonoid glycosides including 4 sulfated derivatives, 2 lignans, and 9 fatty acids. Fr 1 showed a promising hepatoprotective activity when tested in-vitro utilizing HepG2 cell lines and in-vivo against thioacetamide-induced liver fibrosis in mice. Ingestion of Fr 1 in the form of a microemulsion had significantly higher oral bioavailability than when ingested as a suspension in water.
Nematallah, Khaled; Ayoub, Nahla A.; Abdelsattar, Essam; Meselhy, Meselhy R.; Elmazar, Mohamed M.; El-Khatib, Ahmed H.; Linscheid, Michael W.; Hathout, Rania M.; Godugu, Kavitha; Adel, Aya; and Mousa, Shaker A., "Polyphenols LC-MS2 profile of Ajwa date fruit (Phoenix dactylifera L.) and their microemulsion: Potential impact on hepatic fibrosis" (2018). Pharmacy. 201.