Optimization Studies and Chemical Investigations of Aspergillus terreus-18 Showing Antioxidant Activity

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©2017 National Information and Documentation Center (NIDOC) Aspergillus liquid chromatography terreus-18 ethyl (HPLC). acetate This extract led was to the chemically isolation analyzed of the butenolide using High butyrolactone performance I (BL-1) which was identified using nuclear magnetic resonance (NMR) and mass spectra. As a major compound, BL-1 was tested for its antioxidant activity through its ability to scavenge the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). It showed high scavenging activity with IC50 439.09 (µg/ml). A. terreus-18 local isolate was chosen among 18 other isolates screened for their DPPH scavenging activities. A.terreus-18 showed highest scavenging activity (% inhibition), 80.4± 1.35. Malt peptone broth was then used as production media where A.terreus-18 was grown under static conditions, hence DPPH scavenging activity reached 85%. Optimization of different physicochemical parameters to enhance bioactivity and increase the total phenolic content (TPC) of the promising isolate was done through response surface methodology (RSM). Statistical approaches led to 1.17 fold increase of DPPH scavenging activity, 99.5% inhibition. This was obtained upon using (g/L) malt extract, 25; peptone, 6 as fermentation media with initial pH, 7 and volume 50% media /flask (50% aeration) and using inoculum size 15% v/v of 60 h aged culture incubated statically for 18 days at temperature 27±2 °C. Extracellular TPC and antioxidant activity positively correlated where TPC increased from 122.475 to 290.51 mg gallic acid/g of tested extract.

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