Title

Decolorization of reactive black 5 by Micrococcus luteus and Candida albicans in wastewaters

Document Type

Article

Publication Date

1-1-2014

Abstract

© IDOSI Publications, 2014. Biological decolorization of dye effluent is receiving much consideration due to cost effective and its safety use. A total of 24 isolates (9 bacteria and 15 fungi) were isolated from Al-Salam textile factory wastewater by the enrichment culture technique, using Reactive Black 5 (RB5) as a sole carbon source. A bacterial isolate (identified as Micrococcus luteus) showed the highest biodegradation activity. This isolate was able to remove up to 66% of the dye within 3 days of incubation. Comparatively, 53 % of the dye was decolourized by a yeast isolate (identified as Candida albicans) after 3 days of incubation. Addition of yeast extract at a concentration of 10 g L1 enhanced the biodegradation, where complete disappearance of the dye was observed within 3 days of incubation. Interestingly, the bacterium was able to decolorize the dye up to a concentration of 40 mg L1. The residual dye was traced and detected by TLC, UV absorbance as well as HPLC, after 5 days of incubation with Micrococcus luteus. The metabolite was detected by TLC with Rf = 0.8 and a wavelength of 597 nm. The bacterium had the capacity to decolorize the dye in industrial wastewaters at a concentration of 10 to 80 mg/L within 7 days of incubation. The cultures could be useful in the treatment of textile dye at manufacturing site fordecolorization of the dye effluent.

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