Title

Approaches to improve the solubility and availability of progesterone biotransformation by Mucor racemosus

Document Type

Article

Publication Date

1-1-2014

Abstract

Substrate solubility in steroid biotransformation is critical for improving the biotransformation of hydrophobic substrates. The present investigation describes the effect of some organic solvents, polyoxyethylene sorbitan monooleate (Tween 80; surfactant) and β-cyclodextrin (β-CD), on the biotransformation of progesterone (PR) by Mucor racemosus. PR was transformed to three derivatives namely; 11α-hydroxyprogesterone (11α-HP) as main product (I), 4-pregnen-18-al-11β, 21-diol-3, 20-dione (Aldosterone) (II) and 20-hydroxy-pregnan-18-oic acid (III) as minor products. Among the tested organic solvents, methanol gave the highest total bioconversion efficiency (T.B.E) of 75.0 ± 2.49%. Tween 80 improved PR biotransformation up to 81.56 ± 2.29%, while with an optimum molar ratio of PR to β-CD of 1:1, the TBE was increased to 87.15 ± 2.17% of the added substrate in presence of β-CD. The interaction between PR and β-CD in the inclusion complex was assessed using differential scanning calorimetry (DSC) and infrared (IR) spectra. © 2014 Informa UK, Ltd.

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