Studies on the production of actinomycin by Nocardioides luteus, a novel source

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Efforts have been devoted to explore the antimicrobial activity of local egyptian actinomycetes isolates. 127 pure actinomycetes isolates were recovered from the stones at the location of the tomb of Tell Basta (the site of the ruins of the old temple of Bast) which is southeast of the Nile Delta at the township of Zagazig in Egypt. The most competent isolate showed promising activity against some pathogenic yeasts, fungi and bacteria. It was identified as Nocardioides luteus. Using 2 ml of 2 days old inoculum of Nocardioides luteus in fermentation medium consisting of (g/l): starch, 10; yeast extract, 4; NaCl, 10; K2HPO4, 2; MgSO4.7H2O, 0.05; CaCO3, 0.02 and FeSO4.7H2O, 0.01, adjusted before autoclaving at pH 7 and incubated for 7 days with shaking rate 200 rpm resulted in maximum antimicrobial activity against Candida albicans ATCC 10231. The minimum inhibitory concentration (MIC) of the culture was determined to be 0.4 μg/ml. Trials have been conducted to separate and identify the active compound(s) responsible for the anticandidal activity. Based on the spectroscopic data from UV, IR, 1H-NMR and 13C-NMR spectra, the isolated compounds were identified as derivatives of actinomycins class. To our knowledge, this is the first time not only to study systematically the bioactivities of Nocardioides luteus; but also to report actinomycin production by a Nocardioides spp. Cytotoxicity of both the culture filtrate extract and the impure compound were tested against different tumor cell lines where they showed remarkable cytotoxicity against all challenged cell lines.

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