Microbial side-chain degradation of progesterone I: Optimization of the transformation conditions
The microbial side-chain degradation of progesterone for the production of C-19 androgens was investigated. Thirty seven locally isolated fungal cultures were screened for their ability to degrade the side-chain of progesterone. Fusarium dimerum showed the greatest bioconversion efficiency and was selected for further studies. 50% of the substrate was converted to androstenedione after 24 hrs. 72 hrs old culture was able to produce maximum yields of testosterone, androstenedione and androstadienedione. The maximum conversion activities (90%) of progesterone were recorded at pH 7. The capacity of the fungus to degrade the side-chain of progesterone was greatly diminished on using high concentration of progesterone. The bioconversion estimates sharply decreased by using glucose syrup, corn steep liquor and glucose-corn steep media.
Adham, Nehad Z.; El-Tayeb, Osama M.; Hashem, Abdel Gawad M.; El-Refai, Heba A.; and Sallam, Lotfy A., "Microbial side-chain degradation of progesterone I: Optimization of the transformation conditions" (2002). Pharmacy. 408.