Cytotoxic compounds from the leaves of Gaillardia aristata Pursh. growing in Egypt

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Ten compounds, neopulchellin (1), 6α-hydroxyneopulchellin (2),β-sitosterol-3-O-β-D-glucoside (3), apigenin (4), quercitin (5), eupafolin (6), kaempferol-3-methoxy-7-O-α-L-rhamnoside (7), apigenin-7-Oβ-D-glucopyranoside (8),α-amyrin (9) and β-sitosterol (10), were isolated from the leaves of Gaillardia aristata by applying bioassay guided fractionation. The cytotoxicity was traced against two human cancer cell lines (breast (MCF7) and colon (HCT116)). The highest cytotoxicity was revealed by compounds 1 and 2 (isolated from chloroform extract); with IC50 values of 0.43, 0.32μgmL-1 against MCF7 and 0.46, 0.34μgmL-1 against HCT116, respectively. Compounds 9 and 10 (isolated from the n-hexane extract) exhibited lower IC50 values of 3.05, 2.35μgmL-1 against MCF7 and 3.05, 2.35μgmL-1 against HCT116, respectively, while compounds 4-7 obtained from the ethyl acetate extract revealed the lowest cytotoxicity. Identification of the aforementioned compounds was carried out on the basis of their physico-chemical properties and spectral analysis (UV, EI/MS, 1D and 2D). © 2012 Taylor & Francis Group, LLC.

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