Immuno-analytical approach and its application for cardiac disease marker detection

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© 2018, © 2018 Taylor & Francis. Cardiac troponin-I is a promising diagnostic marker for cardiovascular diseases. Troponin-I immunoassays rely on monoclonal antibodies, while polyclonal antibodies, cheaper to manufacture, are uncommonly used. The current study established an immuno-analytical assay using a polyclonal antibody capable of mapping troponin-I antigenic determinant. Proteolytic digestion of troponin-I was performed. Antigenic determinant was assigned by separation of fragments using gel electrophoresis followed by Western blot and high-performance liquid chromatography followed by dot blot. The antigenic determinant region appeared within amino acid sequence 30–90. This robust procedure is suitable for early prognosis of diseases, stratification of patients, and possibly individualized therapy.

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