Document Type


Publication Date



20-Hydroxyecdysone (20-HE) was detected by TLC in the ethyl acetate fraction of two Atriplex species grown in Egypt: A. lindleyi subsp. inflata and A. leucoclada. EIA quantification of 20-HE proved its presence in a concentration of 9.15 and 7.3 μg/g dried aerial parts in each of the two species, respectively. Significant anabolic and androgenic activities were demonstrated for 20-HE comparing to testosterone; the study also revealed that the total alcohol extract and the ethyl acetate fraction of A. lindleyi subsp. inflata exhibited greater activities than their analogues in A. leucoclada. Column chromatographic fractionation of the ethyl acetate fraction of the most active species A. lindleyi subsp. inflata resulted in the isolation of 20-HE (I) in addition to three flavonol glycosides: Isorhamnetin 3-O- galactoside (II), Isoquercitin (III) and Rutin (IV) identified via spectral analysis. DNA were amplified using seven decamer primers as a contribution to the characterization and discrimination between the two Atriplex species indicating only one polymorphic band between the two species and displaying a similarity coefficient of 88.88%.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.