Salvadora persica L. (S. persica, Siwak) has been used for many centuries as oral hygiene tools, particularly in Saudi Arabia. This study aimed to assess the eﬀectiveness of S. persica petroleum ether extract (SPE) as an intracanal bactericidal for endodontictreatmentagainstEnterococcusfaecalis.CalciumhydroxideCa(OH)2 gold standard intracanal medicament was used for comparison.Methods.Thegaschromatographymassspectrometry(GC/MS)analysis was carried out to identify the components of SPE. First, the consistency of SPE was accomplished according to ANSI/ADA speciﬁcation no 57. Forty-ﬁve single-rooted mandibular premolars were infected with that of E. faecalis suspension. Colony-forming units (CFU) were counted before the medicaments’ application (CFU-1) and after seven days of their applications (CFU-2). Group I: SPE, Group II: positive control Ca(OH)2,and group iii:saline solution negative control.Themicrodilutionmethodwasappliedtodetermineminimalinhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of SPE. Results. Thirty-two compounds were identiﬁed (89.09%), with main components of benzyl isothiocyanate(BITC)(33.32%) and steroids(34%).CFUbeforeandafterusingSPEand Ca(OH)2 recorded a statistically signiﬁcant reduction in bacterial count (P � 0.006) and (P � 0.01), respectively. There was an insigniﬁcantdiﬀerencebetweenCFUafterusingSPEandCa(OH)2(P � 0.210).On the contrary,comparing both medicaments with thenegativecontrolsalinegroupresultedinsigniﬁcantdiﬀerences,(P � 0.001)and(P � 0.007),respectively.Moreover,the equality of minimum bactericidal concentration(MBC)and minimum inhibitory concentration(MIC) ofSPEisrecorded.Conclusion.This ﬁnding could be referred to as the high content of bactericidal BITC in synergism with other antimicrobial components, representing 70.71% of SPE. Thus, SPE is a good candidate as an intracanal medicament, which warrants further investigation.
Swilam, Noha; Ayoub, Nahla; Badr, Nadia; Al-Ghamdi,, Saeed S; Alzahrani, Arwa; Alsulaimani, Rahaf; Nassar, Afnan; and Qadi, Rawabi, "GC/MSProfilingandExVivoAntibacterialActivityofSalvadora persica (Siwak) against Enterococcus faecalis as Intracanal Medicament" (2021). Pharmacy. 579.