Electrospun Poly-Lactic Acid Nanofibers for Dual Drug Delivery in Regenerative endodontics: Ciprofloxacin and Metronidazole Loading and Characterization

Document Type

Article

Publication Date

6-2025

Abstract

Aim: This study presents the synthesis, material characterization, and evaluation of the antimicrobial activity of antibiotic-loaded

scaffolds.

Materials and methods: Metronidazole (MID) or Ciprofloxacin (CIP) was incorporated into a polylactic acid (PLA) polymer

solution at a concentration of 25 wt.% and then processed into Fibers. Neat PLA nanofibers were used as controls. Characterization

techniques, including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), degradation tests, and

drug release assessments, were employed. The antimicrobial properties were tested against Porphyromonasgingivalis (Pg) and

Enterococcus faecalis (Ef).

Results: SEM imaging showed Fibers with submicron diameters, and FTIR confirmed the successful incorporation of the antibiotics.

Data analysis demonstrated a gradual and sustained release of the drugs from the scaffolds over 48 hours. Antimicrobial activity was

confirmed via agar diffusion assays, showing efficacy against the tested bacteria.

Conclusion: Antibiotic-loaded electrospun PLA scaffolds could serve as biologically safe antimicrobial drug delivery systems for use in regenerative endodontics.

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