Document Type
Article
Publication Date
6-17-2024
Abstract
This work explores the fabrication route of curcumin-loaded carboxymethyl cellulose sodium/sodium alginate/palladium chloride (CMC-Na/SA/PdCl2) composite hydrogel as localized delivery system for cancer treatment. Herein, an approach was established for improving encapsulation capacity and exploiting therapeutic efficiency. CMC-Na/SA/PdCl2 composite hydrogels were prepared by gelation method using ionic crosslinker (PdCl2). Results proved that the optimized formula (CMC-Na:SA (4:1)/PdCl2 0.4%) recorded a gel fraction of 91% with a swelling ratio ~ 3000% after 96 h, while XRD analysis exhibited no remarks of sharp peaks which confirmed an amorphous phase of tested hydrogel. TGA results showed that this recipe is thermostable compared to other tested formulae. Moreover, SEM of crosslinked unloaded and curcumin-loaded hydrogel showed interconnected pores indicating the interior crosslinked chains formed. According to FT-IR analysis, curcumin was incorporated successfully into CMC-Na/SA via intermolecular hydrogen bonding between curcumin and hydrogel components. In vitro cytotoxicity of CMC-Na/SA/PdCl2 hydrogel exhibited an inhibition proliferation for breast cancer cells (MDA-MB231), liver cancer cells (HePG-2), and colon cancer cells (CaCo-2) without any toxic effect on the normal cells. Additionally, curcumin-loaded CMC-Na/SA/PdCl2 composite hydrogels exhibited only 50% inhibition proliferation for HePG-2 with 0.6% curcumin. Also, curcumin-free crosslinked CMC-Na/SA (4:1) hydrogels exhibited 80% inhibition proliferation for HePG-2 cells. According to antimicrobial bioassay data, CMC-Na:SA (4:1)/PdCl2 0.4%/0.6% Cr hydrogel has the maximum biofilm inhibition as was recorded against Staphylococcus aureus(77%), followed by Bacillus cereus(74%) and Candida krusei (66%). Thus, CMC-Na/SA/PdCl2 composite hydrogels could be regarded as promising antibacterial and anticancer biomaterials for multipurpose biomedical applications
Recommended Citation
salim, samar A.; A Kamoun, Elbadawy; emam, mariam; H El-Moslamy, Shahira; elsawaf, ayman; nassar, amal; and eldeeb, nehal, "Fabrication of Metallo-Pharmaceutical Composite Hydrogel Composed of Curcumin-Loaded CMC-Na/Sodium Alginate/PdCl2: Optimization, Antimicrobial Activity, and Cancer Cell Mortality In Vitro Assessment" (2024). Nanotechnology Research Centre. 112.
https://buescholar.bue.edu.eg/nanotech_research_centre/112