Document Type

Article

Publication Date

Winter 12-24-2025

Abstract

This study endeavours to craft a potent topical facial mask using electrospun nanofibers. The nanofibers were carefully fabricated based on loading of N-acetylcysteine (NAC) onto electrospun marine collagen (MC), and poly (methyl methacrylate) (PMMA) nanofibers. Through precise adjustments of electrospinning parameters, a uniform array of nanoscale fibres with a smooth surface was achieved. The morphology and chemical structure configuration of nanofibers (NFs) were examined using SEM, FTIR, and XRD analyses. Furthermore, the cytotoxic impact was assessed on a human normal fibroblast cell line (BJ1), while protein levels were gauged using the Bradford dye-binding assay (Coomassie assay). Also, the antioxidant potential of various NFs scaffolds was evaluated through superoxide dismutase activity (SOD), catalase activity (CAT), and free radical scavenging assays. Controlled release of NAC from nanofibers was observed. Notably, PMMA/MC/NAC NFs exhibited notable antioxidant and strong antagonistic antimicrobial effects. After an 8-h treatment period, Staphylococcus epidermidis showed the highest percentage of biofilm reduction (96.46 ± 2.09 %) caused by this formula. Moreover, this formula effectively decreased the biofilm production of Candida glabrata (97.18 ± 0.48 %) and Klebsiella pneumoniae (96.38 ± 2.47 %) after a 12-h exposure period. These compelling findings put PMMA/MC/ NAC NFs as promising candidate for facial masks and skincare formulations.

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