An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity

Document Type

Article

Publication Date

10-21-2016

Abstract

© 2016 The Royal Society of Chemistry. The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer. Having improved the chemical synthesis and purification of the fluorescently-labelled DMB-DP3 acceptor, we report optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human polysialyltransferase activity.

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