An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity
© 2016 The Royal Society of Chemistry. The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer. Having improved the chemical synthesis and purification of the fluorescently-labelled DMB-DP3 acceptor, we report optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human polysialyltransferase activity.
Elkashef, Sara M.; Sutherland, Mark; Patterson, Laurence H.; Loadman, Paul M.; and Falconer, Robert A., "An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity" (2016). Pharmacy. 361.