Enhancement of some natural antioxidants activity via microbial bioconversion process using gamma irradiation and incorporation into gold nanoparticles

Document Type

Article

Publication Date

10-2-2012

Abstract

Aspergillum royal was used to enhance the mobilization of antioxidants of soybean matrix along with garlic as a co-substrate by modulating polyphenolic substances during solid-state fermentation. The levels of [3-glucosiclase enzyme, total phonemic content, DPPH free radical scavenging activity and reducing power were investigated during fermentation. Exposure of fungal cultures to gamma irradiation at a dose of 0.75 key yielded relative increase of DPPH free radical scavenging activity, reducing power and total phenol content by about 10%, 54% and 29%, respectively compared to un-irradiated cultures. The antioxidant activity was optimized employing factorial design. The variables evaluated were; pH, temperature, incubation period, inoculums size and humidity, with optimal conditions of 7, 35°C, 6 days, 9 ×l0 6spores/ml and 60%, respectively, with DPPH free radical scavenging activity as response. The maximum DDPH free radical scavenging activity obtained was 95.4%. The most significant variables reported were moisture content and temperature. The aqueous fermented extract was used for rapid preparation of gold Nan particles (AuNPs) confirmed by UV-Vis spectrometry, dynamic light scattering, FT-IR spectrometry and TEM analysis which revealed morphology of spherical AuNPs with size ranging from 7-20 nm. Mobilized polyphonies are suggested to be responsible for synthesis and stabilization of gold Nan particles. The reaction was rapid and completed within one minute. © IDOSI Publications, 2012.

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