Document Type
Article
Publication Date
1-1-2017
Abstract
© Copyright 2017 Wafaa A. Zaghary et al. New HPLC-UV method (method A), for simultaneous determination of metformin (MET) and canagliflozin (CANA), was developed and compared to another novel UPLC-UV method (method B) in their tablet combination. Concerning method A, isocratic separation was done by C18 column (100mm × 2.1 mm, 3 μm) using methanol and 0.03M phosphate buffer (75: 25, V/V) at pH 3.2 as a mobile phase. Meanwhile, chromatographic separation in method B was achieved via Hypersil® gold (50mm × 2.1 mm, 1.9 μm). Mobile phase was methanol and 0.03M phosphate buffer at ratio of 80: 20 V/V. In both, detection was done at wavelength of 240 nm. Method A showed satisfactory linearity results over 1-50 μg · mL-1 and 0.5-100 μg · mL-1, while method B linearitywas at 0.1-50 μg · mL-1 and 0.25-100 μg · mL-1 for CANAandMET, respectively. In terms of accuracy and precision, method A accuracy was 99.81 ± 0.73 and 99.37 ± 0.54, while method B gave accuracy of 99.47 ± 1.03 and 99.73 ± 0.89 for CANA andMET, respectively. For precision, the % RSD was found to be less than 2% for three concentrations analyzed three times.The two methods are convenient for quality laboratories, yet the UPLC method offered the advantage of shorter run times and higher sensitivity.
Recommended Citation
Zaghary, Wafaa A.; Mowaka, Shereen; and Hendy, Moataz S., "Comparative liquid chromatographic study for concurrent determination of canagliflozin and metformin in combined tablets" (2017). Pharmacy. 53.
https://buescholar.bue.edu.eg/pharmacy/53