© Copyright 2017 Wafaa A. Zaghary et al. New HPLC-UV method (method A), for simultaneous determination of metformin (MET) and canagliflozin (CANA), was developed and compared to another novel UPLC-UV method (method B) in their tablet combination. Concerning method A, isocratic separation was done by C18 column (100mm × 2.1 mm, 3 μm) using methanol and 0.03M phosphate buffer (75: 25, V/V) at pH 3.2 as a mobile phase. Meanwhile, chromatographic separation in method B was achieved via Hypersil® gold (50mm × 2.1 mm, 1.9 μm). Mobile phase was methanol and 0.03M phosphate buffer at ratio of 80: 20 V/V. In both, detection was done at wavelength of 240 nm. Method A showed satisfactory linearity results over 1-50 μg · mL-1 and 0.5-100 μg · mL-1, while method B linearitywas at 0.1-50 μg · mL-1 and 0.25-100 μg · mL-1 for CANAandMET, respectively. In terms of accuracy and precision, method A accuracy was 99.81 ± 0.73 and 99.37 ± 0.54, while method B gave accuracy of 99.47 ± 1.03 and 99.73 ± 0.89 for CANA andMET, respectively. For precision, the % RSD was found to be less than 2% for three concentrations analyzed three times.The two methods are convenient for quality laboratories, yet the UPLC method offered the advantage of shorter run times and higher sensitivity.
Zaghary, Wafaa A.; Mowaka, Shereen; and Hendy, Moataz S., "Comparative liquid chromatographic study for concurrent determination of canagliflozin and metformin in combined tablets" (2017). Pharmacy. 53.