Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage

Authors

Amira Khalil, 1Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada. Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt, El Sherouk, EgyptFollow
Mirat Sojitra, Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
Jasmine Maghera, Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
Emily Rodrigues, Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
Eric J. Carpenter, Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
Shaurya Seth, Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
Daniel Ferrer Vinals, Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
Todd L. Lowary, Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada

Document Type

Article

Publication Date

7-2021

Abstract

The central dogma of biology does not allow for the study of glycans using DNA sequencing. We report a liquid glycan array (LiGA) platform comprising a library of DNA ‘barcoded’ M13 virions that display 30–1,500 copies of glycans per phage. A LiGA is synthesized by acylation of the phage pVIII protein with a dibenzocyclooctyne, followed by ligation of azido-modified glycans. Pulldown of the LiGA with lectins followed by deep sequencing of the barcodes in the bound phage decodes the optimal structure and density of the recognized glycans. The LiGA is target agnostic and can measure the glycan-binding profile of lectins, such as CD22, on cells in vitro and immune cells in a live mouse. From a mixture of multivalent glycan probes, LiGAs identify the glycoconjugates with optimal avidity necessary for binding to lectins on living cells in vitro and in vivo.

Recommended Citation

Khalil, Amira; Sojitra, Mirat; Maghera, Jasmine; Rodrigues, Emily; Carpenter, Eric J.; Seth, Shaurya; Vinals, Daniel Ferrer; and Lowary, Todd L., "Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage" (2021). Pharmacy. 604.
https://buescholar.bue.edu.eg/pharmacy/604