Novel Optical Biosensor Based on a Nano-Gold Coated by Schiff Base Doped in Sol/Gel Matrix for Sensitive Screening of Oncomarker CA-125

Authors

Ekram Hany Mohamed, The British University in EgyptFollow
Mohamed S. S. Attia, Chemistry Department, Faculty of Science, Ain Shams University, Abbassia, Cairo, 11566, Egypt
Mona N. Abou-Omar,, Department of Chemistry, Ain Shams University, Faculty of Women for Arts, Science and Education, Cairo, Egypt
Mohammed A. Amin,, Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia
Rabah Boukherroub, Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520, IEMN, Lille, F-59000, France
hisham afify, Department of Chemistry, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, 13013, Egypt

Document Type

Article

Publication Date

Summer 8-17-2021

Abstract

The urge for sensitive, facile, minimally invasive, and fast detection method of CA-125, a significant and crucial biomarker in ovarian malignancy, is currently substantial. This paper describes the detailed construction and characterization of a newly designed optical nano-biosensor to detect CA-125 accurately and sensitively. The fabricated sensor consists of a nano-gold thin film doped into a matrix of sol-gel, exhibiting a centered fluorescence band at 423 nm when excited at 340 nm. The quantification of CA-125 relies on its quenching ability of this fluorescence signal. The sensor was challenged to evaluate its sensitivity and specificity in detecting CA-125 present in samples collected from ovarian cancer diagnosed patients and compared to samples from healthy women as a control. Our findings revealed that the developed biosensor had a sensitivity of 97.35% and a specificity of 94.29%. Additionally, a wide linearity range over 2.0-127.0 U mL-1 for CA-125 was achieved with a detection limit of 1.45 U mL-1. Furthermore, the sensor could successfully discriminate samples between healthy and diseased people, which demonstrates its suitability in CA-125 assessment.

Recommended Citation

Mohamed, Ekram Hany; Attia, Mohamed S. S.; Abou-Omar,, Mona N.; Amin,, Mohammed A.; Boukherroub, Rabah; and afify, hisham, "Novel Optical Biosensor Based on a Nano-Gold Coated by Schiff Base Doped in Sol/Gel Matrix for Sensitive Screening of Oncomarker CA-125" (2021). Pharmacy. 696.
https://buescholar.bue.edu.eg/pharmacy/696