Tb3+-atorvastatin doped in poly(ethylene glycol) optical biosensor for selective determination of progesterone and testosterone in serum samples

Authors

Ekram Hany Mohamed, The British University in EgyptFollow
Mohamed S. S. Attia, Chemistry Department, Faculty of Science, Ain Shams University, Abbassia, Cairo, 11566, Egypt
Safwat A. Mahmoud,, Physics Department, Faculty of Science, Northern Border University, Arar, Saudi Arabia
Amal M. Ahmed,, Chemistry Department, Faculty of Science, Ain Shams University, Cairo, 11566, Egypt
Tarek A. Amin, Chemistry Department, Faculty of Science, Ain Shams University, Cairo, 11566, Egypt
Ahmed O. Youssef, Analytical Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Mona N. Abou-Omar,, Department of Chemistry, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt
Mohammed A. c Amin,, Department of Chemistry, Collage of Science, Taif University, P. O. Box 11099, Taif, 21944, Saudi Arabia

Document Type

Article

Publication Date

Fall 9-27-2021

Abstract

An innovative, simple and cost effective Tb3+-atorvastatin photo probe was designed and used as a core for a spectrofluorometric approach to sensitively determine two vital biological compounds in serum samples. Tb3+-atorvastatin complex displays a characteristic electrical band with λem at 545 nm with significant luminescence intensity, which is quenched in the presence of progesterone and testosterone at two variant sets of pH; 6.2 and 7.5, respectively. The conditions were optimized and the best solvent for operation was found to be acetonitrile with λex at 320 nm. Progesterone and testosterone were assessed in serum samples using the same optimal conditions within concentration ranges of 2 × 10-9 to 2.9 × 10-6 and 3.1 × 10-9 to 4.8 × 10-6 mol L-1, respectively. The proposed luminescence method was validated in accordance to ICH guidelines and found to be accurate, precise and specific and free from any interference. The cost effectiveness and applicability of the method make it a good choice for routine analysis of the two compounds and early diagnosis of chronic diseases associated with abnormalities in their physiological levels.

Recommended Citation

Mohamed, Ekram Hany; Attia, Mohamed S. S.; Mahmoud,, Safwat A.; Ahmed,, Amal M.; Amin, Tarek A.; Youssef, Ahmed O.; Abou-Omar,, Mona N.; and Amin,, Mohammed A. c, "Tb3+-atorvastatin doped in poly(ethylene glycol) optical biosensor for selective determination of progesterone and testosterone in serum samples" (2021). Pharmacy. 699.
https://buescholar.bue.edu.eg/pharmacy/699