Novel hybrid nanofibers for burn wounds: Fucoidan-coated cefdinir nanoparticles in PVA matrices

Document Type

Article

Publication Date

Summer 6-21-2025

Abstract

Nanofiber-based drug delivery systems hold significant potential for wound care due to their large surface area, porosity, and ability to deliver drugs in a controlled manner. This study aimed to develop and evaluate a novel hybrid nanofibers (NFs) mat combining Fucoidan-coated cefdinir-loaded Chitosan nanoparticles with polyvinyl alcohol (PVA) for enhanced burn wound healing. The hybrid NFs were fabricated using electrospinning and evaluated for physicochemical properties, in vitro drug release, ex vivo skin permeation, skin deposition, and burn wound healing efficacy. Swelling and weight loss studies were also evaluated, which highlighted the hydration and degradation profiles. Healing progression was assessed through the in vitro scratch wound assay and the in vivo murine burn wound healing model, supported by histopathological and immunohistochemical analyses. Fucoidan-coated cefdinir-loaded Chitosan nanoparticles incorporated into polyvinyl alcohol nanofibers (Fu-cCFD-CSNPs-PVA NFs) exhibited uniform, smooth, bead-free morphology with an average diameter of 295.12 nm ± 48.52 nm, optimal swelling capacity of 250.41 % ± 9.59, and controlled degradation of 48.55 % ± 1.44. The system demonstrated sustained cefdinir release of 84.00 % ± 2.23 within 48 h as well as enhanced ex vivo skin permeation, deposition, and antimicrobial efficacy. The in vitro studies showed improved human skin fibroblast proliferation and migration. In addition, the in vivo evaluations revealed accelerated wound closure with superior tissue regeneration in the murine burn wound healing model. Histopathological and immunohistochemical analyses confirmed enhanced TGF-β1 expression and organized collagen deposition. This multifunctional delivery system offers controlled drug release kinetics combined with the therapeutic benefits of Fucoidan, presenting an advanced approach for burn wound management. Thus, the developed hybrid NFs show potential as a novel antimicrobial burn wound dressing for local therapy.

Link to Full Text

Share

COinS