Synthesis of nucleoside 5′-tetraphosphates containing terminal fluorescent labels via activated cyclic trimetaphosphate
Document Type
Article
Publication Date
3-7-2014
Abstract
2′-Deoxynucleotide 5′-tetraphosphates in which a fluorescent label is attached to the terminal phosphate are used as key reagents in high-throughput DNA sequencing techniques and in single nucleotide polymorphism typing assays. We demonstrate that this class of compounds can be prepared by reacting fluorophores such as 7-hydroxy-4-methylcoumarin, methylfluorescein, fluorescein and resorufin with an activated form of cyclic trimetaphosphate to give intermediate 11. Reaction of 11 with 2′-deoxynucleoside 5′-monophosphates or a nucleoside 5′-monophosphate gave the target compounds in good yield. © 2014 American Chemical Society.
Recommended Citation
Mohamady, Samy and Taylor, Scott D., "Synthesis of nucleoside 5′-tetraphosphates containing terminal fluorescent labels via activated cyclic trimetaphosphate" (2014). Pharmacy. 92.
https://buescholar.bue.edu.eg/pharmacy/92